Ischemia – Modified albumin by albumin cobalt binding test: a false myth or reality

Abstract Scientific knowledge should be based on evidence. However, some scientific studies can carried out without sufficient And these may mislead subsequent studies. Ischemia-modified albumin (IMA) is a relatively newly proposed biomarker. It used not only for myocardial ischemia but also other pathological conditions in the body. IMA commonly measured by cobalt binding (ACB) assay. ACB simple colorimetric assay performed patients’ sera. claimed that because of or ischemia-reperfusion, molecular structure N-terminus human serum changed and therefore it cannot bind metal ions added into reaction mixture react with dithiothreitol to give brown color. The clinical performance poor has strong correlation biomarkers. There are many analytical uncertainties as well. Despite uncertainties, still research Therefore theory questioned. In this opinion paper, we discussed uncertainties. conclusion, there insufficient evidence existence essentially measures concentration. interfering factors Therefore, measurement any condition useless effort.